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human fibroblast growth factor 2  (R&D Systems)


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    R&D Systems human fibroblast growth factor 2
    Human Fibroblast Growth Factor 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 899 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+fgf+2/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pm41978430-44-25-30
    Average 96 stars, based on 899 article reviews
    human fibroblast growth factor 2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Modulation of fibronectin extracellular matrix enhances anti-tumor efficacy of immune checkpoint blockade.
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) were cultured in EndoGro VEGF (Millipore # SCME002). .. Mouse Lung endothelial cells (mLungECs, mVera-Lng-01, Angiocrine Biosciences), were cultured on 0.1% gelatin coated plates in advanced DMEM/F12 (Sigma Aldrich) with 20% FCS, 1% L-glutamine, 1% penicillin, 1% streptomycin, 10 mM HEPES pH 7.0, 50 μg/mL heparin, MEM non-essential amino acids (Gibco), 50 μg/mL endothelial cell growth supplement (ECGS) (Alfa Aesar, cat no # AAJ64516MF), 20 ng/mL recombinant human FGF-2 (R&D Systems, 233-FB) and 10 ng/mL of recombinant human VEGF-A165 (R&D Systems # 293-VE). .. E0771 (female, also referred to as EO771 C57BL/6, ATCC, RRID: CVCL_GR23), EMT6 (female, BALB/c ATCC, RRID: CVCL_1923) EMT6-CDDP (female, from B. Teicher35), 66cL4 (female, also referred to as 66cL-4 RRID:CVCL_9721) and CT26 MNNG+ EMS+ were cultured in DMEM with 5% FCS.

    Article Title: Modulation of fibronectin extracellular matrix enhances anti-tumor efficacy of immune checkpoint blockade
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) were cultured in EndoGro VEGF (Millipore # SCME002). .. Mouse Lung endothelial cells (mLungECs, mVera-Lng-01, Angiocrine Biosciences), were cultured on 0.1% gelatin coated plates in advanced DMEM/F12 (Sigma Aldrich) with 20% FCS, 1% L-glutamine, 1% penicillin, 1% streptomycin, 10 mM HEPES pH 7.0, 50 μg/mL heparin, MEM non-essential amino acids (Gibco), 50 μg/mL endothelial cell growth supplement (ECGS) (Alfa Aesar, cat no # AAJ64516MF), 20 ng/mL recombinant human FGF-2 (R&D Systems, 233-FB) and 10 ng/mL of recombinant human VEGF-A 165 (R&D Systems # 293-VE). .. E0771 (female, also referred to as EO771 C57BL/6, ATCC, RRID: CVCL_GR23), EMT6 (female, BALB/c ATCC, RRID: CVCL_1923) EMT6-CDDP (female, from B. Teicher ), 66cL4 (female, also referred to as 66cL-4 RRID:CVCL_9721) and CT26 MNNG + EMS + were cultured in DMEM with 5% FCS.

    Recombinant:

    Article Title: Modulation of fibronectin extracellular matrix enhances anti-tumor efficacy of immune checkpoint blockade.
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) were cultured in EndoGro VEGF (Millipore # SCME002). .. Mouse Lung endothelial cells (mLungECs, mVera-Lng-01, Angiocrine Biosciences), were cultured on 0.1% gelatin coated plates in advanced DMEM/F12 (Sigma Aldrich) with 20% FCS, 1% L-glutamine, 1% penicillin, 1% streptomycin, 10 mM HEPES pH 7.0, 50 μg/mL heparin, MEM non-essential amino acids (Gibco), 50 μg/mL endothelial cell growth supplement (ECGS) (Alfa Aesar, cat no # AAJ64516MF), 20 ng/mL recombinant human FGF-2 (R&D Systems, 233-FB) and 10 ng/mL of recombinant human VEGF-A165 (R&D Systems # 293-VE). .. E0771 (female, also referred to as EO771 C57BL/6, ATCC, RRID: CVCL_GR23), EMT6 (female, BALB/c ATCC, RRID: CVCL_1923) EMT6-CDDP (female, from B. Teicher35), 66cL4 (female, also referred to as 66cL-4 RRID:CVCL_9721) and CT26 MNNG+ EMS+ were cultured in DMEM with 5% FCS.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy.
    Article Snippet: The neural induction medium consisted of DMEM-F12 with 1% N2 supplement, 1mM nonessential amino acids (NEAAs), and 2mM glutamate (all from ThermoFisher Scientific). .. The neural induction factors for CHSF, namely, 3 μM CHIR99021 (Cayman Chemical), 10μM SB431542 (Sigma-Aldrich), and 10 ng/mL recombinant human FGF-2 (R&D Systems, Minneapolis, MN, USA), were added on days 1 to 4 without changing the medium. .. On day 4, the neural induction media were changed to neurobasal media (ThermoFisher Scientific) containing 1% N2 supplement, 1mM NEAAs, 2mM glutamate, and MNP patterning factors (3μM CHIR99021, 2μM SB431542, and 200 nM LDN193189; Cayman Chemical), 0.5 μM SAG (Cayman Chemical), and 0.5μM RA (Sigma-Aldrich) until day 25 of differentiation.

    Article Title: Modulation of fibronectin extracellular matrix enhances anti-tumor efficacy of immune checkpoint blockade
    Article Snippet: Human umbilical vein endothelial cells (HUVECs) were cultured in EndoGro VEGF (Millipore # SCME002). .. Mouse Lung endothelial cells (mLungECs, mVera-Lng-01, Angiocrine Biosciences), were cultured on 0.1% gelatin coated plates in advanced DMEM/F12 (Sigma Aldrich) with 20% FCS, 1% L-glutamine, 1% penicillin, 1% streptomycin, 10 mM HEPES pH 7.0, 50 μg/mL heparin, MEM non-essential amino acids (Gibco), 50 μg/mL endothelial cell growth supplement (ECGS) (Alfa Aesar, cat no # AAJ64516MF), 20 ng/mL recombinant human FGF-2 (R&D Systems, 233-FB) and 10 ng/mL of recombinant human VEGF-A 165 (R&D Systems # 293-VE). .. E0771 (female, also referred to as EO771 C57BL/6, ATCC, RRID: CVCL_GR23), EMT6 (female, BALB/c ATCC, RRID: CVCL_1923) EMT6-CDDP (female, from B. Teicher ), 66cL4 (female, also referred to as 66cL-4 RRID:CVCL_9721) and CT26 MNNG + EMS + were cultured in DMEM with 5% FCS.

    Article Title: Replenishable prevascularized cell encapsulation devices increase graft survival and function in the subcutaneous space.
    Article Snippet: INSGFP/W hESCs were cultured on irradiated mouse embryonic fibroblasts (Thermo Fisher) in hESC maintenance media composed of Dulbecco's Modified Eagle Medium (DMEM)/F12, 20% (vol/vol) KnockOut serum replacement (Thermo Fisher Scientific), nonessential amino acids (Thermo Fisher Scientific), GlutaMAX (Thermo Fisher Scientific), and 2-mercaptoethanol (Millipore). .. The maintenance media was supplemented with 4 ng/mL recombinant human FGF-2 (R&D Systems). .. Confluent hESCs were dissociated into single-cell suspension by incubation with TrypLE Select (Gibco) and passaged every 3–4 days.

    Article Title: Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy
    Article Snippet: The neural induction medium consisted of DMEM-F12 with 1% N2 supplement, 1 mM nonessential amino acids (NEAAs), and 2 mM glutamate (all from ThermoFisher Scientific). .. The neural induction factors for CHSF, namely, 3 μM CHIR99021 (Cayman Chemical), 10 μM SB431542 (Sigma-Aldrich), and 10 ng/mL recombinant human FGF-2 (R&D Systems, Minneapolis, MN, USA), were added on days 1 to 4 without changing the medium. .. On day 4, the neural induction media were changed to neurobasal media (ThermoFisher Scientific) containing 1% N2 supplement, 1 mM NEAAs, 2 mM glutamate, and MNP patterning factors (3 μM CHIR99021, 2 μM SB431542, and 200 nM LDN193189; Cayman Chemical), 0.5 μM SAG (Cayman Chemical), and 0.5 μM RA (Sigma-Aldrich) until day 25 of differentiation.

    Article Title: Coacervate-Filled Lipid Vesicles for Protein Delivery.
    Article Snippet: Macromolecularly crowded coacervate is useful in protein delivery for tissue engineering and regenerative medicine.. However, coacervate tends to aggregate easily, which impedes their application.. Here, this work presents a method to prepare coacervate with enhanced stability.

    Article Title: Replenishable prevascularized cell encapsulation devices increase graft survival and function in the subcutaneous space
    Article Snippet: INS GFP/W hESCs were cultured on irradiated mouse embryonic fibroblasts (Thermo Fisher) in hESC maintenance media composed of Dulbecco's Modified Eagle Medium (DMEM)/F12, 20% (vol/vol) KnockOut serum replacement (Thermo Fisher Scientific), nonessential amino acids (Thermo Fisher Scientific), GlutaMAX (Thermo Fisher Scientific), and 2‐mercaptoethanol (Millipore). .. The maintenance media was supplemented with 4 ng/mL recombinant human FGF‐2 (R&D Systems). .. Confluent hESCs were dissociated into single‐cell suspension by incubation with TrypLE Select (Gibco) and passaged every 3–4 days.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Coacervate-Filled Lipid Vesicles for Protein Delivery.
    Article Snippet: Macromolecularly crowded coacervate is useful in protein delivery for tissue engineering and regenerative medicine.. However, coacervate tends to aggregate easily, which impedes their application.. Here, this work presents a method to prepare coacervate with enhanced stability.



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    R&D Systems basic fibroblast growth factor 2
    Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; <t>FGF2.</t> Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.
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    Image Search Results


    Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; FGF2. Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.

    Journal: Military Medical Research

    Article Title: Engineering of functional auditory neurons from human induced pluripotent stem cells

    doi: 10.1016/j.mmr.2026.100008

    Figure Lengend Snippet: Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; FGF2. Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.

    Article Snippet: On D3, the medium was replaced with a CDM containing 10 ng/ml bone morphogenetic protein 4 (BMP4; Cat# 314-BP, R&D Systems, USA), 1 μmol/L SB-431542 (Cat# 1614, TOCRIS, UK), and 10 ng/ml basic fibroblast growth factor 2 (FGF2, Cat# 233-FB, R&D Systems, USA).

    Techniques: Comparison, Immunostaining, Expressing, Staining, Derivative Assay, Marker, Standard Deviation

    Origin of human SGN-like neurons revealed by scRNA-seq. a Schematic illustration of the developmental origins of auditory neurons in the human inner ear in vivo . b UMAP plots of SK8-A D25 cells, color-coded as in  a. c Feature plots showing key gene markers for the classification of cell subtypes and determination of neuronal origins. d Re-clustering of isolated otic neural primordium cells from  b, separating into three populations: EMT, delaminating neuroblasts, and neurogenesis. e Heatmap showing the top 100 differentially expressed genes across the three distinctive clusters: EMT, NB, and NG. The X and Y axes represent differentially expressed genes and single cells, respectively. f Violin plots displaying marker gene expression in a log-transformed scale among the three different clusters of otic neural primordium. The number in the upper right corner represents log-normalized gene expression (log1p normalized UMI counts) per cell. g Feature plot visualizing expression of SGN and VGN markers in the otic neural primordium cluster, based on scRNA-seq data from previous studies  . Numbers in the lower left corners indicate the percentage of cells expressing each marker. h Dot plots illustrating minimal expression of hindbrain, GG, and EPI markers, and high expression of otic neuroblast and neuronal markers. To support data sharing, visualization, and analysis, the processed scRNA-seq dataset for  is available on the gEAR portal ( https://umgear.org/p?l=sk8aNeuron ). SGN. Spiral ganglion neuron; scRNA-seq. Single-cell RNA sequencing; UMAP. Uniform manifold approximation and projection; EMT. Epithelial-mesenchymal transition; NB. Neuroblasts; NG. Neurogenesis; VGN. Vestibular ganglion neurons; GG. Geniculate ganglion; EPI. Epibranchial placode; PLP1. Proteolipid protein 1; TWIST1. Twist family bHLH transcription factor 1; TOP2A. DNA topoisomerase II alpha; MEUROD1. Neuronal differentiation 1; SOX2. SRY-box transcription factor 2; WNT4. Wnt family member 4; FGF8. Fibroblast growth factor 8; DCX. Doublecortin; MEIS2. Meis homeobox 2; PROX1. Prospero homeobox 1; TLX3. T cell leukemia homeobox 3; SALL3. Spalt like transcription factor 3; PVALB. Parvalbumin; CNC. Cranial neural crest; ZBTB16. Zinc finger and BTB domain containing 16; PAX6. Paired box 6; HOXA2. Homeobox A2; FOXI1. Forkhead box I1; PHOX2A. Paired mesoderm homeobox protein 2A; FOXI2. Forkhead box I2; NEUROG1. Neurogenin 1; INSM1. Insulinoma-associated 1; HE56. Hes family BHLH transcription factor 6; MAP2. Microtubule-associated protein 2; TUBB3. Tubulin beta 3 class III.

    Journal: Military Medical Research

    Article Title: Engineering of functional auditory neurons from human induced pluripotent stem cells

    doi: 10.1016/j.mmr.2026.100008

    Figure Lengend Snippet: Origin of human SGN-like neurons revealed by scRNA-seq. a Schematic illustration of the developmental origins of auditory neurons in the human inner ear in vivo . b UMAP plots of SK8-A D25 cells, color-coded as in a. c Feature plots showing key gene markers for the classification of cell subtypes and determination of neuronal origins. d Re-clustering of isolated otic neural primordium cells from b, separating into three populations: EMT, delaminating neuroblasts, and neurogenesis. e Heatmap showing the top 100 differentially expressed genes across the three distinctive clusters: EMT, NB, and NG. The X and Y axes represent differentially expressed genes and single cells, respectively. f Violin plots displaying marker gene expression in a log-transformed scale among the three different clusters of otic neural primordium. The number in the upper right corner represents log-normalized gene expression (log1p normalized UMI counts) per cell. g Feature plot visualizing expression of SGN and VGN markers in the otic neural primordium cluster, based on scRNA-seq data from previous studies . Numbers in the lower left corners indicate the percentage of cells expressing each marker. h Dot plots illustrating minimal expression of hindbrain, GG, and EPI markers, and high expression of otic neuroblast and neuronal markers. To support data sharing, visualization, and analysis, the processed scRNA-seq dataset for is available on the gEAR portal ( https://umgear.org/p?l=sk8aNeuron ). SGN. Spiral ganglion neuron; scRNA-seq. Single-cell RNA sequencing; UMAP. Uniform manifold approximation and projection; EMT. Epithelial-mesenchymal transition; NB. Neuroblasts; NG. Neurogenesis; VGN. Vestibular ganglion neurons; GG. Geniculate ganglion; EPI. Epibranchial placode; PLP1. Proteolipid protein 1; TWIST1. Twist family bHLH transcription factor 1; TOP2A. DNA topoisomerase II alpha; MEUROD1. Neuronal differentiation 1; SOX2. SRY-box transcription factor 2; WNT4. Wnt family member 4; FGF8. Fibroblast growth factor 8; DCX. Doublecortin; MEIS2. Meis homeobox 2; PROX1. Prospero homeobox 1; TLX3. T cell leukemia homeobox 3; SALL3. Spalt like transcription factor 3; PVALB. Parvalbumin; CNC. Cranial neural crest; ZBTB16. Zinc finger and BTB domain containing 16; PAX6. Paired box 6; HOXA2. Homeobox A2; FOXI1. Forkhead box I1; PHOX2A. Paired mesoderm homeobox protein 2A; FOXI2. Forkhead box I2; NEUROG1. Neurogenin 1; INSM1. Insulinoma-associated 1; HE56. Hes family BHLH transcription factor 6; MAP2. Microtubule-associated protein 2; TUBB3. Tubulin beta 3 class III.

    Article Snippet: On D3, the medium was replaced with a CDM containing 10 ng/ml bone morphogenetic protein 4 (BMP4; Cat# 314-BP, R&D Systems, USA), 1 μmol/L SB-431542 (Cat# 1614, TOCRIS, UK), and 10 ng/ml basic fibroblast growth factor 2 (FGF2, Cat# 233-FB, R&D Systems, USA).

    Techniques: In Vivo, Isolation, Marker, Gene Expression, Transformation Assay, Expressing, Single Cell, RNA Sequencing